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ubxn3b  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc ubxn3b
    ( a ) Immunoprecipitation (IP) of FLAG-APEX-caspase-4 with an anti-FLAG antibody. HeLa cells were transfected with a FLAG-APEX-CASP4 and/or HA-Ub (WT) plasmid for 24 h, primed with IFN-γ for 12 h and/or transfected with LPS for 5 h. (-) stands for an empty vector plasmid. Shown are the immunoblots (IB) of indicated proteins. WCL, whole cell lysate; KO, knockout of UBXN1 . ( b ) IP of FLAG-APEX-caspase-4 with an anti-FLAG antibody from HeLa cells transfected with different combinations of FLAG-APEX-CASP4, Myc-UBXN1, <t>Myc-UBXN3B</t> and corresponding vector (-) for 24 h, followed by treatment with (+) / without (-) human IFN-γ and LPS as in ( a ). The endogenous Ub were examined by polyubiquitin antibodies. ( c ) IP of FLAG-APEX-caspase-4 with an anti-FLAG antibody from HeLa cells transfected with various combinations of FLAG-APEX-CASP4, Myc-UBXN1, HA-tagged WT or individual Kn-Ub (mutant) plasmids. (-) stands for an empty vector plasmid. ( d ) IP of FLAG-caspases with an anti-FLAG antibody from HeLa cells transfected with a FLAG-CASP, Myc-UBXN1 or empty vector plasmid. The red arrow heads indicate correct bands; caspase-11 shows in two bands. The endogenous total, K48, and K63 Ub were detected by specific antibodies. ( e ) IP of FLAG-caspase-4 and Myc-UBXN1 from HEK293T cells. Cells were transfected with the FLAG-CASP4, Myc-UBXN1 or both for 24 h; the cell lysates were equally split for IP with an anti-Myc and anti-FLAG antibody separately. The endogenous K48- and K63-Ub were detected by Ub linkage specific antibodies. Immunoblots (IB) in ( a - e ) shows the indicated proteins detected with specific antibodies. WCL, whole cell lysate.
    Ubxn3b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ubxn3b/ETEA%2FUBXD8+Rabbit+mAb/bio_rxiv__2024__10__30__621131-204-34-77
    Average 93 stars, based on 7 article reviews
    ubxn3b - by Bioz Stars, 2026-10
    93/100 stars

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    1) Product Images from "Unanchored ubiquitin chains promote the non-canonical inflammasome via UBXN1"

    Article Title: Unanchored ubiquitin chains promote the non-canonical inflammasome via UBXN1

    Journal: bioRxiv

    doi: 10.1101/2024.10.30.621131

    ( a ) Immunoprecipitation (IP) of FLAG-APEX-caspase-4 with an anti-FLAG antibody. HeLa cells were transfected with a FLAG-APEX-CASP4 and/or HA-Ub (WT) plasmid for 24 h, primed with IFN-γ for 12 h and/or transfected with LPS for 5 h. (-) stands for an empty vector plasmid. Shown are the immunoblots (IB) of indicated proteins. WCL, whole cell lysate; KO, knockout of UBXN1 . ( b ) IP of FLAG-APEX-caspase-4 with an anti-FLAG antibody from HeLa cells transfected with different combinations of FLAG-APEX-CASP4, Myc-UBXN1, Myc-UBXN3B and corresponding vector (-) for 24 h, followed by treatment with (+) / without (-) human IFN-γ and LPS as in ( a ). The endogenous Ub were examined by polyubiquitin antibodies. ( c ) IP of FLAG-APEX-caspase-4 with an anti-FLAG antibody from HeLa cells transfected with various combinations of FLAG-APEX-CASP4, Myc-UBXN1, HA-tagged WT or individual Kn-Ub (mutant) plasmids. (-) stands for an empty vector plasmid. ( d ) IP of FLAG-caspases with an anti-FLAG antibody from HeLa cells transfected with a FLAG-CASP, Myc-UBXN1 or empty vector plasmid. The red arrow heads indicate correct bands; caspase-11 shows in two bands. The endogenous total, K48, and K63 Ub were detected by specific antibodies. ( e ) IP of FLAG-caspase-4 and Myc-UBXN1 from HEK293T cells. Cells were transfected with the FLAG-CASP4, Myc-UBXN1 or both for 24 h; the cell lysates were equally split for IP with an anti-Myc and anti-FLAG antibody separately. The endogenous K48- and K63-Ub were detected by Ub linkage specific antibodies. Immunoblots (IB) in ( a - e ) shows the indicated proteins detected with specific antibodies. WCL, whole cell lysate.
    Figure Legend Snippet: ( a ) Immunoprecipitation (IP) of FLAG-APEX-caspase-4 with an anti-FLAG antibody. HeLa cells were transfected with a FLAG-APEX-CASP4 and/or HA-Ub (WT) plasmid for 24 h, primed with IFN-γ for 12 h and/or transfected with LPS for 5 h. (-) stands for an empty vector plasmid. Shown are the immunoblots (IB) of indicated proteins. WCL, whole cell lysate; KO, knockout of UBXN1 . ( b ) IP of FLAG-APEX-caspase-4 with an anti-FLAG antibody from HeLa cells transfected with different combinations of FLAG-APEX-CASP4, Myc-UBXN1, Myc-UBXN3B and corresponding vector (-) for 24 h, followed by treatment with (+) / without (-) human IFN-γ and LPS as in ( a ). The endogenous Ub were examined by polyubiquitin antibodies. ( c ) IP of FLAG-APEX-caspase-4 with an anti-FLAG antibody from HeLa cells transfected with various combinations of FLAG-APEX-CASP4, Myc-UBXN1, HA-tagged WT or individual Kn-Ub (mutant) plasmids. (-) stands for an empty vector plasmid. ( d ) IP of FLAG-caspases with an anti-FLAG antibody from HeLa cells transfected with a FLAG-CASP, Myc-UBXN1 or empty vector plasmid. The red arrow heads indicate correct bands; caspase-11 shows in two bands. The endogenous total, K48, and K63 Ub were detected by specific antibodies. ( e ) IP of FLAG-caspase-4 and Myc-UBXN1 from HEK293T cells. Cells were transfected with the FLAG-CASP4, Myc-UBXN1 or both for 24 h; the cell lysates were equally split for IP with an anti-Myc and anti-FLAG antibody separately. The endogenous K48- and K63-Ub were detected by Ub linkage specific antibodies. Immunoblots (IB) in ( a - e ) shows the indicated proteins detected with specific antibodies. WCL, whole cell lysate.

    Techniques Used: Immunoprecipitation, Transfection, Plasmid Preparation, Western Blot, Knock-Out, Mutagenesis

    Related Articles

    Hemagglutination Assay:

    Article Title: Unanchored ubiquitin chains promote the non-canonical inflammasome via UBXN1
    Article Snippet: Antibodies for GAPDH (Cat# 60004-1-Ig, 1:2000), UBXN1 (Cat# 16135-1-AP, 1:1000), UBXN6 (Cat# 14706-1-AP, 1:1000), GBP1 (Cat# 15303-1-AP, 1:1000) were purchased from Proteintech Group. .. Caspase-4 (Cat# 4450S, 1:1000), Gasdermin D (Cat# 39754S, 1:1000), Cleaved Gasdermin D (Asp275) (Cat# 36425S, 1:500), Cleaved Gasdermin D (Asp276) (Cat# 10137S, 1:1000), NLRP3 (Cat# 15101S, 1:1000), Tubulin (Cat# 2144S, 1:2000), Actin (Cat#4967S, 1:2000), UBXN3B (Cat# 34945S, 1:1000), K63-linkage specific polyubiquitin (Clone D7A11, Cat# 5621S,1:500), K48-linkage specific polyubiquitin (Clone D9D5, Cat# 12805S, 1:1000), Phospho-Jak1 (Tyr1034/1035) (Cat# 74129S, 1:1000), Phospho-Stat1 (Tyr701) (Cat# 9167S, 1:1000), Myc-Tag (Cat# 2276S, 1:2000) and HA-Tag (Clone C29F4, Cat# 3724S, 1:1000) antibodies were from Cell Signaling. .. Caspase-1 (Cat# ab179515, 1:1000), Caspase-11 (Cat# ab180673, 1:1000), GSDMD (Cat# ab209845, 1:1000) antibodies and recombinant human USP5 protein (Active) (IsoT, Cat# ab269124) were from Abcam.

    Cell-Signaling:

    Article Title: Unanchored ubiquitin chains promote the non-canonical inflammasome via UBXN1
    Article Snippet: Antibodies for GAPDH (Cat# 60004-1-Ig, 1:2000), UBXN1 (Cat# 16135-1-AP, 1:1000), UBXN6 (Cat# 14706-1-AP, 1:1000), GBP1 (Cat# 15303-1-AP, 1:1000) were purchased from Proteintech Group. .. Caspase-4 (Cat# 4450S, 1:1000), Gasdermin D (Cat# 39754S, 1:1000), Cleaved Gasdermin D (Asp275) (Cat# 36425S, 1:500), Cleaved Gasdermin D (Asp276) (Cat# 10137S, 1:1000), NLRP3 (Cat# 15101S, 1:1000), Tubulin (Cat# 2144S, 1:2000), Actin (Cat#4967S, 1:2000), UBXN3B (Cat# 34945S, 1:1000), K63-linkage specific polyubiquitin (Clone D7A11, Cat# 5621S,1:500), K48-linkage specific polyubiquitin (Clone D9D5, Cat# 12805S, 1:1000), Phospho-Jak1 (Tyr1034/1035) (Cat# 74129S, 1:1000), Phospho-Stat1 (Tyr701) (Cat# 9167S, 1:1000), Myc-Tag (Cat# 2276S, 1:2000) and HA-Tag (Clone C29F4, Cat# 3724S, 1:1000) antibodies were from Cell Signaling. .. Caspase-1 (Cat# ab179515, 1:1000), Caspase-11 (Cat# ab180673, 1:1000), GSDMD (Cat# ab209845, 1:1000) antibodies and recombinant human USP5 protein (Active) (IsoT, Cat# ab269124) were from Abcam.

    Immunofluorescence:

    Article Title: UBXN3B positively regulates STING-mediated antiviral immune responses
    Article Snippet: .. The rabbit anti-GAPDH (D16H11, Cat# 5174, 1:3000), calreticulin (D3E6, Cat# 12238, 1:200 for IFA), α-Tubulin (Cat# 2144, 1:3000), UBXN3B (D8H6D, Cat# 34945, 1:1000 for IB, 1:100 for IFA), TBK1 (D1B4, Cat# 3504, 1:1000), phospho-TBK1 (D52C2, Cat# 5483, 1:500), K63-linked polyubiquitin (D7A11, Cat# 5621,1:500), phospho-IRF3 (4D4G, Cat# 4947, 1:500), human phospho-STING (Cat# 85735, 1:500), and STING (D2P2F, Cat#13647, 1:500) were purchased from Cell Signaling Technology (Danvers, MA, USA). .. The goat anti-TRIM56 (E-18, Cat# sc-249085, 1:200) was obtained from Santa Cruz Biotechnology (CA, USA).



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    ( a ) Immunoprecipitation (IP) of FLAG-APEX-caspase-4 with an anti-FLAG antibody. HeLa cells were transfected with a FLAG-APEX-CASP4 and/or HA-Ub (WT) plasmid for 24 h, primed with IFN-γ for 12 h and/or transfected with LPS for 5 h. (-) stands for an empty vector plasmid. Shown are the immunoblots (IB) of indicated proteins. WCL, whole cell lysate; KO, knockout of UBXN1 . ( b ) IP of FLAG-APEX-caspase-4 with an anti-FLAG antibody from HeLa cells transfected with different combinations of FLAG-APEX-CASP4, Myc-UBXN1, <t>Myc-UBXN3B</t> and corresponding vector (-) for 24 h, followed by treatment with (+) / without (-) human IFN-γ and LPS as in ( a ). The endogenous Ub were examined by polyubiquitin antibodies. ( c ) IP of FLAG-APEX-caspase-4 with an anti-FLAG antibody from HeLa cells transfected with various combinations of FLAG-APEX-CASP4, Myc-UBXN1, HA-tagged WT or individual Kn-Ub (mutant) plasmids. (-) stands for an empty vector plasmid. ( d ) IP of FLAG-caspases with an anti-FLAG antibody from HeLa cells transfected with a FLAG-CASP, Myc-UBXN1 or empty vector plasmid. The red arrow heads indicate correct bands; caspase-11 shows in two bands. The endogenous total, K48, and K63 Ub were detected by specific antibodies. ( e ) IP of FLAG-caspase-4 and Myc-UBXN1 from HEK293T cells. Cells were transfected with the FLAG-CASP4, Myc-UBXN1 or both for 24 h; the cell lysates were equally split for IP with an anti-Myc and anti-FLAG antibody separately. The endogenous K48- and K63-Ub were detected by Ub linkage specific antibodies. Immunoblots (IB) in ( a - e ) shows the indicated proteins detected with specific antibodies. WCL, whole cell lysate.
    Ubxn3b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ubxn3b/ETEA%2FUBXD8+Rabbit+mAb/bio_rxiv__2024__10__30__621131-204-34-77
    Average 93 stars, based on 1 article reviews
    ubxn3b - by Bioz Stars, 2026-10
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    ( a ) Immunoprecipitation (IP) of FLAG-APEX-caspase-4 with an anti-FLAG antibody. HeLa cells were transfected with a FLAG-APEX-CASP4 and/or HA-Ub (WT) plasmid for 24 h, primed with IFN-γ for 12 h and/or transfected with LPS for 5 h. (-) stands for an empty vector plasmid. Shown are the immunoblots (IB) of indicated proteins. WCL, whole cell lysate; KO, knockout of UBXN1 . ( b ) IP of FLAG-APEX-caspase-4 with an anti-FLAG antibody from HeLa cells transfected with different combinations of FLAG-APEX-CASP4, Myc-UBXN1, <t>Myc-UBXN3B</t> and corresponding vector (-) for 24 h, followed by treatment with (+) / without (-) human IFN-γ and LPS as in ( a ). The endogenous Ub were examined by polyubiquitin antibodies. ( c ) IP of FLAG-APEX-caspase-4 with an anti-FLAG antibody from HeLa cells transfected with various combinations of FLAG-APEX-CASP4, Myc-UBXN1, HA-tagged WT or individual Kn-Ub (mutant) plasmids. (-) stands for an empty vector plasmid. ( d ) IP of FLAG-caspases with an anti-FLAG antibody from HeLa cells transfected with a FLAG-CASP, Myc-UBXN1 or empty vector plasmid. The red arrow heads indicate correct bands; caspase-11 shows in two bands. The endogenous total, K48, and K63 Ub were detected by specific antibodies. ( e ) IP of FLAG-caspase-4 and Myc-UBXN1 from HEK293T cells. Cells were transfected with the FLAG-CASP4, Myc-UBXN1 or both for 24 h; the cell lysates were equally split for IP with an anti-Myc and anti-FLAG antibody separately. The endogenous K48- and K63-Ub were detected by Ub linkage specific antibodies. Immunoblots (IB) in ( a - e ) shows the indicated proteins detected with specific antibodies. WCL, whole cell lysate.
    Rabbit Anti Ubxn3b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( a ) Immunoprecipitation (IP) of FLAG-APEX-caspase-4 with an anti-FLAG antibody. HeLa cells were transfected with a FLAG-APEX-CASP4 and/or HA-Ub (WT) plasmid for 24 h, primed with IFN-γ for 12 h and/or transfected with LPS for 5 h. (-) stands for an empty vector plasmid. Shown are the immunoblots (IB) of indicated proteins. WCL, whole cell lysate; KO, knockout of UBXN1 . ( b ) IP of FLAG-APEX-caspase-4 with an anti-FLAG antibody from HeLa cells transfected with different combinations of FLAG-APEX-CASP4, Myc-UBXN1, Myc-UBXN3B and corresponding vector (-) for 24 h, followed by treatment with (+) / without (-) human IFN-γ and LPS as in ( a ). The endogenous Ub were examined by polyubiquitin antibodies. ( c ) IP of FLAG-APEX-caspase-4 with an anti-FLAG antibody from HeLa cells transfected with various combinations of FLAG-APEX-CASP4, Myc-UBXN1, HA-tagged WT or individual Kn-Ub (mutant) plasmids. (-) stands for an empty vector plasmid. ( d ) IP of FLAG-caspases with an anti-FLAG antibody from HeLa cells transfected with a FLAG-CASP, Myc-UBXN1 or empty vector plasmid. The red arrow heads indicate correct bands; caspase-11 shows in two bands. The endogenous total, K48, and K63 Ub were detected by specific antibodies. ( e ) IP of FLAG-caspase-4 and Myc-UBXN1 from HEK293T cells. Cells were transfected with the FLAG-CASP4, Myc-UBXN1 or both for 24 h; the cell lysates were equally split for IP with an anti-Myc and anti-FLAG antibody separately. The endogenous K48- and K63-Ub were detected by Ub linkage specific antibodies. Immunoblots (IB) in ( a - e ) shows the indicated proteins detected with specific antibodies. WCL, whole cell lysate.

    Journal: bioRxiv

    Article Title: Unanchored ubiquitin chains promote the non-canonical inflammasome via UBXN1

    doi: 10.1101/2024.10.30.621131

    Figure Lengend Snippet: ( a ) Immunoprecipitation (IP) of FLAG-APEX-caspase-4 with an anti-FLAG antibody. HeLa cells were transfected with a FLAG-APEX-CASP4 and/or HA-Ub (WT) plasmid for 24 h, primed with IFN-γ for 12 h and/or transfected with LPS for 5 h. (-) stands for an empty vector plasmid. Shown are the immunoblots (IB) of indicated proteins. WCL, whole cell lysate; KO, knockout of UBXN1 . ( b ) IP of FLAG-APEX-caspase-4 with an anti-FLAG antibody from HeLa cells transfected with different combinations of FLAG-APEX-CASP4, Myc-UBXN1, Myc-UBXN3B and corresponding vector (-) for 24 h, followed by treatment with (+) / without (-) human IFN-γ and LPS as in ( a ). The endogenous Ub were examined by polyubiquitin antibodies. ( c ) IP of FLAG-APEX-caspase-4 with an anti-FLAG antibody from HeLa cells transfected with various combinations of FLAG-APEX-CASP4, Myc-UBXN1, HA-tagged WT or individual Kn-Ub (mutant) plasmids. (-) stands for an empty vector plasmid. ( d ) IP of FLAG-caspases with an anti-FLAG antibody from HeLa cells transfected with a FLAG-CASP, Myc-UBXN1 or empty vector plasmid. The red arrow heads indicate correct bands; caspase-11 shows in two bands. The endogenous total, K48, and K63 Ub were detected by specific antibodies. ( e ) IP of FLAG-caspase-4 and Myc-UBXN1 from HEK293T cells. Cells were transfected with the FLAG-CASP4, Myc-UBXN1 or both for 24 h; the cell lysates were equally split for IP with an anti-Myc and anti-FLAG antibody separately. The endogenous K48- and K63-Ub were detected by Ub linkage specific antibodies. Immunoblots (IB) in ( a - e ) shows the indicated proteins detected with specific antibodies. WCL, whole cell lysate.

    Article Snippet: Caspase-4 (Cat# 4450S, 1:1000), Gasdermin D (Cat# 39754S, 1:1000), Cleaved Gasdermin D (Asp275) (Cat# 36425S, 1:500), Cleaved Gasdermin D (Asp276) (Cat# 10137S, 1:1000), NLRP3 (Cat# 15101S, 1:1000), Tubulin (Cat# 2144S, 1:2000), Actin (Cat#4967S, 1:2000), UBXN3B (Cat# 34945S, 1:1000), K63-linkage specific polyubiquitin (Clone D7A11, Cat# 5621S,1:500), K48-linkage specific polyubiquitin (Clone D9D5, Cat# 12805S, 1:1000), Phospho-Jak1 (Tyr1034/1035) (Cat# 74129S, 1:1000), Phospho-Stat1 (Tyr701) (Cat# 9167S, 1:1000), Myc-Tag (Cat# 2276S, 1:2000) and HA-Tag (Clone C29F4, Cat# 3724S, 1:1000) antibodies were from Cell Signaling.

    Techniques: Immunoprecipitation, Transfection, Plasmid Preparation, Western Blot, Knock-Out, Mutagenesis